The Petrifilm Aerobic Count plate is a reliable, ready made medium system for enumerating total aerobic bacteria populations (1,2,3,4). Petrifilm Aerobic Count plates contain Standard Methods nutrients, a cold water soluble gelling agent and a tetrazolium indicator dye which facilitates colony enumeration.
Store sealed Petrifilm Aerobic Count plate foil pouches at or below 46F (8C). After opening, return unused plates to foil pouch. Seal foil pouch by folding and taping the open end. Store resealed foil pouches in a cool dry place. Use plates within one month after opening. Exposure of Petrifilm Aerobic Count plates to temperatures and/or humidities above 75F (24C) and 50% RH can affect the performance of the plates. Resealed foil pouches may be stored in freezer to protect plates from high temperatures and/or humidities. Do not use plates that show brown discoloration.
Work area, level table with ample surface in room that is clean, well-lighted (100 foot-candles at working surface) and well-ventilated, and reasonably free of dust and drafts. The microbial density of air in working area, measured in fallout pour plates taken during plating, should not exceed 15 colonies/plate during 15 min exposure.
What is total aerobic count of bacteria?
Petrifilm® 3M
Aerobic Count Plates
To be used for total aerobic bacteria enumeration.
Description:-
The Petrifilm Aerobic Count plate is a reliable, ready made medium system for enumerating total aerobic bacteria populations (1,2,3,4). Petrifilm Aerobic Count plates contain Standard Methods nutrients, a cold water soluble gelling agent and a tetrazolium indicator dye which facilitates colony enumeration.
Warning:-
Petrifilm Aerobic Count plates are not intended to be used for in vitro diagnostic use.
Storage and disposal:-
Store sealed Petrifilm Aerobic Count plate foil pouches at or below 46F (8C). After opening, return unused plates to foil pouch. Seal foil pouch by folding and taping the open end. Store resealed foil pouches in a cool dry place. Use plates within one month after opening. Exposure of Petrifilm Aerobic Count plates to temperatures and/or humidities above 75F (24C) and 50% RH can affect the performance of the plates. Resealed foil pouches may be stored in freezer to protect plates from high temperatures and/or humidities. Do not use plates that show brown discoloration.
After use, Petrifilm Aerobic Count plates will contain viable bacteria. Handle and discard appropriately.
Directions for use:-
1. Place the Petrifilm Aerobic Count plate on a flat surface.
2. Lift top film. Hold pipette perpendicular to the plate and carefully dispense 1 ml of sample onto the center of bottom film.
3. Release top film down onto sample.
4. Distribute sample evenly using a gentle downward pressure on the center of the (recessed side). Do not slide the spreader across the film. Remove spreader and leave plate undisturbed for ore minute to permit solidification of the gel.
5. Incubate plates in a horizontal position, with the clear side up in stacks not exceeding 20 plates. Follow current total plate count standards for incubation temperature. Temperatures above 37C are not recommended. Incubate plates 48 + 3 hr.
6. Petrifilm Aerobic Count plates can be counted on a standard colony counter. The reduction of the tetrazolium indicator dye will cause the colonies to become red. All red dots regardless of size or intensity should be counted as colonies.
The circular growth area is approximately 20 cm2. Estimates can be made on plates containing greater than 250 colonies by counting a representative number of squares and multiplying by the appropriate number to obtain an estimated count for the total 20 cm2 growth area. The presence of very high concentrations of colonies on me plates will cause the entire growth area to become red or pink in color; record results as "too numerous to count (TNTC). Occasionally, on overcrowded plates, the center may lack visible colonies but many small colonies will be seen on the edges. When this occurs, record results as TNTC. Some organisms can liquefy the gel, allowing them to spread out and obscure the presence of other colonies. If a liquifier interferes with counting, an estimated count should be made by counting the unaffected areas.
7. To isolate colonies for further identification, lift the top film and pick the colony from the gel..
Reply:total aerobic count of bacteria is the count of the both live %26amp; dead bacteria can grow in presence of Oxygen.
it can be done by: 2 methods.
(1)direct method: 1. breeds methd
2.wright M.
3.counter counter
4. membrane filtration
(2)indirect method: 1.brown's method
2.colorymetry
3.nephelometry
4.packed cell volume
5.dry wt. method.
Reply:The Petrifilm Aerobic Count plate is a reliable, ready made medium system for enumerating total aerobic bacteria populations (1,2,3,4). Petrifilm Aerobic Count plates contain Standard Methods nutrients, a cold water soluble gelling agent and a tetrazolium indicator dye which facilitates colony enumeration.
Store sealed Petrifilm Aerobic Count plate foil pouches at or below 46F (8C). After opening, return unused plates to foil pouch. Seal foil pouch by folding and taping the open end. Store resealed foil pouches in a cool dry place. Use plates within one month after opening. Exposure of Petrifilm Aerobic Count plates to temperatures and/or humidities above 75F (24C) and 50% RH can affect the performance of the plates. Resealed foil pouches may be stored in freezer to protect plates from high temperatures and/or humidities. Do not use plates that show brown discoloration.
Work area, level table with ample surface in room that is clean, well-lighted (100 foot-candles at working surface) and well-ventilated, and reasonably free of dust and drafts. The microbial density of air in working area, measured in fallout pour plates taken during plating, should not exceed 15 colonies/plate during 15 min exposure.
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