Do you know what species of bacteria this might be?

It is most likely within the genus Bacillus





catalase +


oxidase -


Gram reaction +


nitrate reduction +


motility -


casein hydrolysis -


lipid hydrolysis +


urea hydrolysis -


starch hydrolysis -


Simmons Citrate +


Kligler's Iron -





I isolated it from tree bark. Colonies are round with a "fried egg" configuration. Light brownish and opaque. Cells are chain-forming rods that contain endospores. It is a facultative anaerobe with a capsule.





I'd really appreciate any suggestions.

Do you know what species of bacteria this might be?
You have a lot of data but you also have a Bacillus that is going to take a bit of research to identify. From the characteristics that you list, I would evaluate the genus Bacillus and look at the subspecies of anthracis or the subspecies cereus. This genus is widespread and found in many habitats. Identification is difficult due to incomplete descriptions of many of the species. I'd refer you to "Bergey's Manual of Systematic Bacteriology". To my knowledge, the data charts you need are not available online. Just about any college with a medical microbiology class will have the book, though. Sorry I can't identify further, but I do believe I've identified the genus for you.





Hope this helps!
Reply:Normally I would not answer such a complicated question. I'm certainly not qualified as a plant specialist.





I did google some key words "fried egg anaerobe simmons citrate +" , and I sort of stuck without an answer too.





I'm wondering, since you are dealing with tree bark, if more than one organism was grown out.





How did you go about growing an isolated bacteria from tree bark?





Perhaps the complexity to your answer lies within your selective media....?





Best,





dumbdumb.





ps. I can't wait for someone to share with us the answer!!!!!

lady slipper