Wednesday, July 21, 2010

How can the cultured staphlococci aureus bacteria be identified on a microscope?

"Staphylococcus aureus forms a fairly large yellow colony on rich medium, S. epidermidis has a relatively small white colony. S. aureus is often hemolytic on blood agar; S. epidermidis is non hemolytic. Staphylococci are facultative anaerobes that grow by aerobic respiration or by fermentation that yields principally lactic acid. The bacteria are catalase-positive and oxidase-negative. S. aureus can grow at a temperature range of 15 to 45 degrees and at NaCl concentrations as high as 15 percent. Nearly all strains of S. aureus produce the enzyme coagulase: nearly all strains of S. epidermidis lack this enzyme. S. aureus should always be considered a potential pathogen; most strains of S. epidermidis are nonpathogenic and may even play a protective role in their host as normal flora. Staphylococcus epidermidis may be a pathogen in the hospital environment.





Staphylococci are perfectly spherical cells about 1 micrometer in diameter. They grow in clusters because staphylococci divide in two planes. The configuration of the cocci helps to distinguish staphylococci from streptococci, which are slightly oblong cells that usually grow in chains (because they divide in one plane only). The catalase test is important in distinguishing streptococci (catalase-negative) from staphylococci, which are vigorous catalase-producers. The test is performed by adding 3% hydrogen peroxide to a colony on an agar plate or slant. Catalase-positive cultures produce O2 and bubble at once. The test should not be done on blood agar because blood itself contains catalase.


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How can the cultured staphlococci aureus bacteria be identified on a microscope?
on a microscope or by a microscope? :P





Golden staph (which is what you're talking about) is easily identifiable- a google image search will bring up some excellent results. Below is an Australian site, have a look around the site, you'll be amazed at how clever us Aussies are ;)





Oh, the link below is for the so-called MRSA "superbug" but you get the idea :)
Reply:Any staph is a Gram positive (violet) group of cocci, not as strep that are in the form of a chain. Culture is the best to difference between one kind or another of staphylococci (epidermidis, aureus, etc)
Reply:Can not be identified using microscope,only by cultering techniques and thrugh biochemical reactions which i see a friend have given you all of it.


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