Monday, November 21, 2011

List the ingredients used to culture bacteria.?

There many different types of cultures and many many many types of bacteria. Depends on what are you looking for.





A microbiological culture, or microbial culture, is a method of growing a microbial organism to determine what it is, its abundance in the sample being tested, or both. It is one of the primary diagnostic methods of microbiology. A tool is often used to determine the cause of infectious disease by letting the agent multiply (reproduce) in predetermined media in laboratory.





The most common method of microbiological culture uses Petri dishes with a layer of agar-based growth medium in them to grow bacterial cultures. This is generally done inside of an incubator. Another method is liquid culture, where the bacteria are grown suspended in a liquid nutrient medium. Bottles of liquid culture are often placed in shakers in order to introduce oxygen to the liquid and maintaining the uniformity of the culture.





The term culture can also, though infrequently and informally, be used as a synonym for tissue culture, which involves the growth of cells or tissues explanted from a multi-cellular organism.

List the ingredients used to culture bacteria.?
A major constituent of the cell walls of certain red algae, especially members of the families Gelidiaceae and Gracilariaceae. Extracted for its gelling properties, it is one of three algal polysaccharides of major economic importance, the others being alginate and carrageenan. Agar is composed of two similar fractions, agarose and agaropectin, in which the basic unit is galactose, linked alternately α-1,3-(D-galactose) and β-1,4-(α-L-galactose).





Agar is prepared by boiling the algae in water, after which the filtered solution is cooled, purified, and dried. It is an amorphous, translucent material that is packaged in granules, flakes, bricks, or sheets. One of its chief uses is as a gelling agent in media for culturing microorganisms. It is also used in making confections, as an emulsifier in cosmetics and food products, as a sizing agent, as an inert carrier of drugs in medicine, and as a laxative.
Reply:Shortly to say (because there are so many various receipes for nutrient media), you need





- a source of carbon


- a source of nitrogen


- any growth factors if needed (amino acids, purines, pyrimidines, vitamins...)


- selective factors to eliminate competing organisms if needed (dyes, antibiotics...)





You also have to supply





- water


- beneficial pH


- oxygen (aerobes) / no oxygen (anaerobes)


- light (photosynthetic organisms) / no light (others)


- beneficial temperature





Please refer some comprehensive mb handbooks or cataloques by nutrient medium suppliers.
Reply:hm, everyone made it sound so complicated, i was just going to say agar (a nutrient medium!)


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