You need to pick (in a sterile manner) some of the bacteria spread from the first agar plate and streak them onto a new agar plate, taking care of turning the plate of a few degrees every so often. To be sure that you are in limiting conditions, which will allow you to end up with individual colonies, you could streak the bacteria a second and third time on sequential plates - therefore performing a "serial dilution" of your bacterial titer. So at least in one of them you will have few enough bacteria to grow sparse and make individual colonies.
You may find a diagram on how it is done here:
http://loudoun.nv.cc.va.us/vetonline/vet...
Can someone explain how to subculture bacteria from colonies on agar plate?
Use a sterile loop to get a sample from the bacteria and streak this sample on to another sterile petri plate.
bleeding heart
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