Monday, November 21, 2011

Is cytochrome c oxidase present in staphylococcus bacteria? please help.?

The enzyme cytochrome c oxidase or Complex IV (PDB 2OCC, EC 1.9.3.1) is a large transmembrane protein complex found in bacteria and the mitochondrion.

Is cytochrome c oxidase present in staphylococcus bacteria? please help.?
yes. go to google and type in staphylococcus.


Which of the following contain more harmful bacteria and why?

The bottom of your shoe, your cell phone, or a toilet seat? First best answer gets 10 points.

Which of the following contain more harmful bacteria and why?
I'm a microbiology student, and I would say your cell phone contains the most harmful bacteria. Sure, you could culture bacteria from the bottom of your shoes, and yes, toilet seats do harbor some bacteria, bust most of it is harmeless. Cell phones probably harbor more pathogenic bacteria. In this respect they are fomites, objects that enable the transmission of bacteria from person to person (like a door knob). You speak into a cell phone, spraying it with countless bacteria from your oral cavity - many of which may be pathogenic and cause colds, throat infections etc. Also, you put your ears right up to cell phones, which would be a great way for bacteria that cause ear infections to be transmitted - they probably thrive since the ear piece and mouth piece of a cell phone are probably the dampest, warmest and most moist area of a cell phone, and many pathogenic bacteria loves such an environment. If people share cell phones, this would be a great way for pathogenic bacteria to be transmitted from person to person. Also, if people in clinics and hospitals use their cell phones there, they may be helping to spread harmful bacteria that way.


Can you get sick from cat litter bacteria?

My girlfriend was scrubbing the dirty litter box in water ,when she finished she realized the rubber glove she was wearing ripped,so that dirty water was soaking into her scrape,she washed her hand very good after she finished,is there anything really dangerous that could happen to her?

Can you get sick from cat litter bacteria?
No.


Mostly germs from our pets don't infect us at all.


Especially in this case.


If she was cleaning she had some sort of cleaner which killed the germs.
Reply:you can get sick from cat litter bacteria espically if the cat using the bo has some kind of sickness or disease on top of that BUT as long as ur girl washed her hands very well with soap and water and the wound is treated she should be fine
Reply:no. pregnant women aren't supposed to clean litter boxes because of the feces, but if she isn't pregnant, then she should be fine. did she have any cuts on her hand?
Reply:Since she washed her hands thoroughly, she should be fine. Pregnant women should not touch cat poop though.
Reply:as long as she is not pregnant cause it can cause birth defects
Reply:If she washed the open wound thoroughly with good soap,she should be fine. Obviously, if she's pregnant, she should get checked out. Keep an eye on it- unless the skin around the scrape has discoloration, pain or swelling, she'll be fine. I put a little neosporin/tripleantibiotic if it's serious cut. Otherwise, a dab of regular iodine.
Reply:She should be fine as long as she washed her hands very well after she finished. Cats can carry a parasite called Toxoplasmosis, which is especially dangerous for pregnant women and puts the fetus at risk, however you have to injest the parasite to get sick. Same thing with any sort of worms or other parasites. Unless she injested any of the feces, she should be ok.
Reply:She could possibly get an infection in the scrape she has. As long as she wash it with soap and water she should be ok. I would recommend cleaning the area with peroxide or rubbing alcohol, then applying first aid cream and it should be just fine.


Pregnant woman should never ever clean a cat litter box.
Reply:UM FIRST OF ALL WHY IS YOUR GIRL FRIEND CLEANING UR LITTER BOX HAHAHAHAHAHA!!!!!!!!!!!!!!!!!!!!!!!!!!!!... yes things can happen there could be life harming conditions she could possibly get ammonia or worse
Reply:She is OK..but if..she wipes her *** and then touches the food that you will eat..you will have a problem!
Reply:there are diseases called zoonotic diseases, these are any that can be transmitted from animal to human or from human to animal. Cats can carry diseases that can make you sick, but it is unlikely. As long as the cat has had all of its shots and it is healthy, your friend is probably fine, just clean the scrape well and use an antiseptic. Always get medical advice from a license physician before you become too nervous about the situation
Reply:Toxoplasmosis is the disease that people can get from cat litter boxes. If your cat has never lived outdoors, never been fed raw meat, never caught or ate any rodents, there should be no risk. Otherwise, you can check out the link I am sending you for further info.


http://www.metrokc.gov/HEALTH/prevcont/t...
Reply:No she should be fine. As long as she's not pregnant as toxoplasmosis could be a problem if she's not already been exposed to it prior to pregnancy! Just keep an eye on it and treat it with antiseptic and if it looks like it's getting worse then off to the Dr. for antibiotics.


What is the function of photosystems in bacteria that have only one of the two Photosystems?

how do these bacteia carry out photosynthesis?

What is the function of photosystems in bacteria that have only one of the two Photosystems?
They carry out the photosynthesis with the help of the photosystem that they have and bacteriochlorophyll
Reply:Can search on yahoo


http://search.yahoo.com/?fr=ks-ans
Reply:They carry out cyclic photosynthesis. The role of the second photosystems in those organisms that have it is to produce NADPH. Those bacteria that only have the one photosystem use reverse electron transport to produce the NADPH, so they get by using just the one photosystem to produce ATP.

yoga

How does simply washing your hands kill bacteria?

hand washing is the first line of defense to prevent the spread of infection is what is taught to all health care professionals


however it is how you wash your hands not just the fact you wash them


hands have to be washed rigorously for at least 3 minutes by the use of soap and water with applied friction


bar soap should be avoided as it is contaminated by previous use and the moisture retained on it allows a medium for bacteria to grow


antibacterial soap helps but is not essential if hands are washed properly


the soap has to be massaged on and around all the fingers(in between also) in brisk motions


also to the tops and palms of the hands and around the wrists


complete rinsing of the area with water and complete drying is essential


you also have to use the paper towels to turn off the faucets


your hands should not come in contact with the faucets or they will be recontaminated

How does simply washing your hands kill bacteria?
it takes away the main bacteria =you have to wash and scrub them though to get rid of almost all the germs
Reply:It doesn't. It will however rinse them away. Though chances are, there are more of them being deposited by the water you are using.
Reply:It doesn't, unless you use scalding hot water. Use an anti-bacterial soap for best results. You need to have your hands under running water for at least 20 seconds, and use a disposable towel to dry, as it doesn't retain germs!
Reply:It works in a couple of different ways. First, it actually washes off some of the bacteria. Second, the scrubbing action actually destroys some of the bacteria by lysing them.
Reply:Okay, the soap that has antibacteral ingredients kills the latent bacteria sitting on your hands, and the friction fron rubbing your hands together kills even more germs. Then, the heat from the water washes them away, while killing some more.
Reply:It does not, it simply removes them. We are all colonized and carriers of common bacteria. Reducing the numbers reduces the statistical chance of getting or passing on an infection.......up to a point. Too much cleanliness clears the way for some other kinds of bacteria to move in.


Alas. it is the Yin and Yang of life.
Reply:It washes away he bacteria...if you use an anti-bacterial soap, that is what kills the bacteria...
Reply:It isn't "simply washing" your hands, it's the amount of TIME u


take to wash them. I figured it out and it should be a long as the tune of Happy Birthday Lyrics. Take it from Health Canada. It's true!








Diana D


How does simply washing your hands kill bacteria?

hand washing is the first line of defense to prevent the spread of infection is what is taught to all health care professionals


however it is how you wash your hands not just the fact you wash them


hands have to be washed rigorously for at least 3 minutes by the use of soap and water with applied friction


bar soap should be avoided as it is contaminated by previous use and the moisture retained on it allows a medium for bacteria to grow


antibacterial soap helps but is not essential if hands are washed properly


the soap has to be massaged on and around all the fingers(in between also) in brisk motions


also to the tops and palms of the hands and around the wrists


complete rinsing of the area with water and complete drying is essential


you also have to use the paper towels to turn off the faucets


your hands should not come in contact with the faucets or they will be recontaminated

How does simply washing your hands kill bacteria?
it takes away the main bacteria =you have to wash and scrub them though to get rid of almost all the germs
Reply:It doesn't. It will however rinse them away. Though chances are, there are more of them being deposited by the water you are using.
Reply:It doesn't, unless you use scalding hot water. Use an anti-bacterial soap for best results. You need to have your hands under running water for at least 20 seconds, and use a disposable towel to dry, as it doesn't retain germs!
Reply:It works in a couple of different ways. First, it actually washes off some of the bacteria. Second, the scrubbing action actually destroys some of the bacteria by lysing them.
Reply:Okay, the soap that has antibacteral ingredients kills the latent bacteria sitting on your hands, and the friction fron rubbing your hands together kills even more germs. Then, the heat from the water washes them away, while killing some more.
Reply:It does not, it simply removes them. We are all colonized and carriers of common bacteria. Reducing the numbers reduces the statistical chance of getting or passing on an infection.......up to a point. Too much cleanliness clears the way for some other kinds of bacteria to move in.


Alas. it is the Yin and Yang of life.
Reply:It washes away he bacteria...if you use an anti-bacterial soap, that is what kills the bacteria...
Reply:It isn't "simply washing" your hands, it's the amount of TIME u


take to wash them. I figured it out and it should be a long as the tune of Happy Birthday Lyrics. Take it from Health Canada. It's true!








Diana D

ivy

Anyways of testing if you've got bacteria in your urine at home?

You can buy test kits without a doctor's order (nonprescription) to use at home to check for urinary tract infections (UTIs). Talk to your doctor about using one of these kits. Make sure that your doctor knows about any abnormal test results so that a urinary problem is not missed.


The urinary tract consists of the kidneys, bladder, ureters, and urethra. Urine in the bladder normally is sterile-it does not contain any bacteria or other organisms (such as fungi). But bacteria can enter the urethra during urination.


Urinary tract infections are more common in women and girls than in men. This may be partly because the female urethra is shorter and closer to the anus, which allows bacteria from the intestines to come into contact more easily with the urethra. (See an illustration of the female urinary tract.) Men also have an antibacterial substance in their prostate gland that reduces their risk.





Two types of home tests can be used to test for a urinary tract infection:


* Dipstick tests. The dipstick test kit contains specially treated plastic strips (dipsticks) that you hold in your urine stream or dip into a sample of your urine. The strips test for a substance (called nitrite) produced by most urinary tract infections. Certain types of strips also test for white blood cells (leukocytes). Some types of dipsticks can test for both nitrite and leukocytes, but most types test for only one or the other. An area on the end of the strip changes color if you have an infection.


* Urine culture. Another type of home test kit is used for growing bacteria that might be in the urine. Special tubes or slides contain a substance (culture medium) that promotes the growth of bacteria. A small sample of urine is placed in the tube or on the slide. If you have an infection, bacteria will grow and become visible on the culture medium.


Most urinary tract infections can be easily cured with antibiotics. However, an untreated infection may spread to the kidneys and cause a more serious problem. If you use a home test kit, make sure that your doctor knows about any abnormal test results so that a serious problem is not missed.

Anyways of testing if you've got bacteria in your urine at home?
try mixing it with soda and see the colour change, if its green it has no bacteria, if pinkinsh then it has some
Reply:there's always gunna be abcteria everywhere...it depends what ur looking for though. if its an std dont be stupid and go to the doctor.
Reply:You don't have bacteria in your urine until you pee. Urine is sterile inside the bladder...once it leaves there it picks up bacteria. so not reallly
Reply:Don't think so, take a sample to your doctor or to doctor's lab.
Reply:No!! It has to be sent to a lab for testing!!
Reply:drink cranberry juice we all have a little bacteria in our urine my grandmother always said drink one beer and it will clean it out. my kidney doctor agrees but I'm not a drinker so i just use cranberry or cranapple juice
Reply:pee into a bowl. If it stings while your doing it then youve got a dose
Reply:Sure, if you want to spend some money and be a scientist. you can stain it and look at it under a microscope, or try to grow a culutre. The easiest and cheapest thing to do is see a doctor.


TRegarding the comment about your urine being sterile: That is only true if YOU DONT HAVE AN INFECTION. If you have a bladder infection, or a kidney infection, your urine is NOT sterile. If you had a bladder infection and they stuck a needle into your bladder to remove some urine, it would have bacteria in it.
Reply:the best way to get rid of it is and keep your body more healthy


drink more water and take


http://www.beauteetdietes.com/products/a...


or u can drink cranberry juice i just rather take caplets its cheaper then buying the juice everyday





i take 2 everyday
Reply:you can buy something called multistix, they are about £15 though. they test for about 12 different things such as lucasytes, protiene, glucose etc. they can detect infections and other abnormalities.it gives you a guideline on the leaflet so you know what your readings should be and you can then compare them.


What are the 3 main types of bacteria and what are their main characteristics ?

1. coccus (comma shaped)


2. bacillus (rods)


3. spirillum(spiral shaped)


Google these names for images.


What are is the growth rate of bacteria measured using a ***** culture that has been incubated for ~16hrs?

Why not at 12 hrs, or 24 hrs, 48 hrs etc. Does the growth rates reach a maximum at 16 hrs?

What are is the growth rate of bacteria measured using a ***** culture that has been incubated for ~16hrs?
Usually at this point the culture is well established, and dilutions will then be made and the optical density of the dilutions can be figured out, which determines the growth rate. After 24 hours cells start to die off b/c of lack of nutrients. After 48 hours, the culture will still be viable but not the best source of robust growth. 16 hours is pretty arbitrary I guess, but it usually represents an overnight incubation, ie incubate at 5pm, come back at 9am.


Differences between DNA replication in eukaryotes and bacteria?

pray tell, any help is reallllllllllly appreciated thank you so much.

Differences between DNA replication in eukaryotes and bacteria?
go here





http://users.rcn.com/jkimball.ma.ultrane...

fuchsia

What are the similarities and differences of bacteria + virus + protists?

Complexity is the difference.


The similarity is that each uses and has the others functions built into the whole, but not separate.


ie a virus uses the protist's shape function to bind rna, create proteins....


bacteria use the virus's rna binding, protein... to replicate it's dna, make metabolites.... function.

What are the similarities and differences of bacteria + virus + protists?
bacteria is living, viruses are not, and protists are worms i think...


Is agar the only medium to culture bacteria?

What is the simplest way of preparing a bacterial culture?

Is agar the only medium to culture bacteria?
i would say get some sterile water (clean water - free from any microorganisms) and add some pure sugar into it !!





here you go !! bacteria will live beutifully there untill the sugar is all used up ...





[dont put too much sugar, as this can kill everything there .. too much sugar will cause the cells to burst, and by the way this is why jams/honey ...etc is hard to grow any bacteria on (except fungi ofcourse)
Reply:Nope. Gelatin is a real good one too. It's also available in a film, cut to fit a petri dish. You need to get your hands on a laboratory supply catalogue.
Reply:The simplest way is probably in a broth: take a colony from an agar plate and dip it into some liquid medium (eg nutrient broth or LB). I think you can also grow it on collagen with a liquid medium, or gelatin.


If flagella are absent, how do theses bacteria move through mammallian tissue?

M. luteus, E. coli, B. subtilis

If flagella are absent, how do theses bacteria move through mammallian tissue?
Bacteria can hitch a ride in the blood stream or lymphatic system, this is called transmigration.


They can live on the surface of your skin and be ingested when you put your fingers in your mouth, which is how your gut is colonized with E. coli when you are a neonate. You can contaminate your other mucous membranes with bacteria, like those lining your mouth, genital region (glans for men, vagina for women), and the like. Bacteria can also be inhaled and transmigrate through the tissues lining your lungs.


Those bacteria that are not scavenged by macrophages, and other immune cells soon set up shop where the environment is cosy, usually on the skin, in mucous membranes (which include the gut) or in the blood stream .


What happens when bioligists find too much bacteria in the water, by the shoreline. What are the risks?

Often, an increase in bacteria in ocean water occurs after rainfall. This is because of an increase in sewage outpouring from treatment plants. the bacteria is usually e. coli or other fecal borne bacterias. The risk is that it can make you sick or die. There are other reasons, though they occur less often, such as a mass death of the creatures living in an area due to polution and warming of the waters which can create a surge of algae growth.

What happens when bioligists find too much bacteria in the water, by the shoreline. What are the risks?
lets see you can catch hepatitis cholera. i live in virginia we have lots of navy ships dumping poop about 5 miles off the coast they shut the beaches down all the time.

paper bush

If tooth decay is caused by a bacteria, why don't dentists prescribe an antibiotic for it?

Listerine can kill the bacteria in your mouth... but you'd have to constantly be swishing with listerine. Antibiotics only help systemic bacteria, and the bacteria in your mouth are topical rather than systemic. Cavities work from the outside of your tooth inward, rather than the other way around.

If tooth decay is caused by a bacteria, why don't dentists prescribe an antibiotic for it?
Because it's not bacteria that is invading INSIDE your body, it's topical bacteria. Antibiotics will do little for that. Plus, if you kill off too much of your beneficial oral bacteria by taking antibiotics, you can develop thrush, or a yeast infection, which is a horrible condition to have. It makes your mucous membranes extremely raw and can actually open you up to a true infection.
Reply:They're probably pissed off that people don't just floss. And think they should suffer the discomfort of having that crap scraped off their teeth and underneath the gums with a sharp pointy object.
Reply:There's probably more to it than this, but you'd have to constantly be taking antibiotics and aside from killing good bacteria, the rate of mutation (and resistance) of the bacteria would be greatly increased. So the initial problem would be unnecessarily be blown up.
Reply:Even if antibiotics could kill all the bacteria in your mouth, once you stop taking the antibiotics, your mouth would soon be invaded by bacteria again. Every person is born with a relatively sterile mouth and all the cavity causing bacteria is transmitted vertically, most commonly parent to child. Another problem, which does happen a lot with people who are on antibiotics too long, is that some of the bacteria in your mouth is beneficial in that it competes with other organisms for resources. Once the bacteria is gone, it'll leave you more vulnerable to fungal infections such as from candida (thrush).





Mouthwashes like listerine do kill many types of disease causing bacteria, but it only works as long as listerine is in your mouth. As soon as you spit it out, it no longer works because it has no staying power. Chlorhexidine is the standard of care by dentists for keeping bacteria levels low if they're going to prescribe a mouthrinse, but the problem is that it will stain your teeth and alter your taste sensation, so it's not usually prescribed for more than 2 weeks at a time.


I want a list of all motile bacteria?

You're on the wrong list - you want "letters-to-santa". Assuming that you've been very good this year, you may find what you want under the tree in a few weeks.


Now, if you were hoping to get some information from people here, a question rather than a demand might get you farther.


Can we isolate any kind of pthogenic bacteria from cashew kernels having moisture between 3% - 5%?

Live bacteria seems unlikely. Bacterial spores, no problems.





You would have more luck trying to isolate fungi.

Can we isolate any kind of pthogenic bacteria from cashew kernels having moisture between 3% - 5%?
Sounds like a great experiment/research topic - good luck because I have no idea. Sorry!
Reply:probably, by spectrophotometer
Reply:dude are you seriously that paranoid that you are skeered to eat yer nuts? i think you need to give up studying bacteria because soon you will be imagining that you have ebola or something. Just eat yer damn nuts and stop worrying so freakin much!


What is the best medicine for pseudomonas bacteria found on wound after operation of hernia ?

what is the side effects of medicine ? what is the best food in such situation

What is the best medicine for pseudomonas bacteria found on wound after operation of hernia ?
You need to be taking an oral antibiotic for this...and it probably wouldn't hurt to use an antibiotic powder when dressing the wound.


Nystatin powder is an example of a topical antibiotic...it is used often for wounds like this...


A culture and sensitivity test would be the best thing to show which antibiotics you need...


You should definetely call the surgeon that did your operation and ask to be seen...


You need to treat this condition with medicine because it could get through to the rest of your organs and get into your blood stream...then it would be septicemia...and you don't want that....


Good luck and I hope you feel better.
Reply:Hi there !


All organisms don't get controlled by all medicines. There will be individual variations, and also it depends upon the site of infection and also on locality.


So the best thing you can do is to get the "Culture and sensitivity" test done on the pus / discharge . Let the "Microbiology department" tell you what "organism" it is and what "drug" the organism will respond to ! okay ?


Different medicines have different mode of action and hence you will have to check that detail from your consultant !





best wishes for a speedy recovery !
Reply:cefnidir or ceftriaxone sulbactum combination.not remarkable.


liquid diet.
Reply:The question for your doctor is, is the pseudomonas on the wound causing an infection or is it colonizing the wound?





Infections from pseudomonas can be bad - there are only a handful of oral medications that can kill it and if the pseudomonas learned to be resistant to them then all you're left with is IV antibiotics.





Colonization, however, occurs when the bacteria is living on the surface but is not penetrating the wound. This is analogous to the bacteria that live on our skin without causing illness. If this is the case, the wound needs to be carefully watched to make sure infection isn't setting in but antibiotics may not necessarily be needed.





Changes in diet won't help with psuedomonas infection.





If you got a culture result back that says pseuodomonas, you need to talk with your doctor and see what s/he thinks it means ASAP.

daphne

Do hamsters carry salmonella or other harmful bacteria like mice do?

Salmonella is more of a concern with reptiles like iguanas and turtles. Hamsters are both rodents, so they should be very similar. Your main concern with them, as with any warm-blooded animal is e. Coli. Just wash your hands after playing with them and you'll be fine.





Most hamsters aren't biters, but be warned, they can bite a lot harder than mice. If you do get bitten, and it breaks the skin, wash it out good and use an antibiotic. Rabies is NOT a concern with rodents - especially a caged rodent (How could it get rabies to start with?)

Do hamsters carry salmonella or other harmful bacteria like mice do?
Please note: Salmonella IS a concern in small animals. Below is a PETA article that states Petsmart sent out a memo in December saying that they were having a salmonella outbreak in several stores. Please make sure to maintain proper hygeine with any animal!


http://www.peta.org/feat-petsm... Report It

Reply:not pet mice
Reply:That's what vaccines exist for.
Reply:yes they do always make sure you wash hands properly and especially children
Reply:I rasie mice and the average cat or dog contains more dieases than7 mice
Reply:I've had hamsters for years and years and read loads of stuff about keeping them, and as far as I'm aware they're really quite clean. They'll carry their own bacteria, like we all do, so wash your hands after you touch them and after cleaning the cage but I've never had any problem whatsoever with anything like that.
Reply:Pet mice do not carry salmonella. untamed rats and mice can but not mice that have been quarantined and sold in a store and neither do pet hamsters or any animal sold from a store. It is possible that they could eventually get some type of decease later on but as long as you keep a healthy and keep his area clean he will be fine.
Reply:My job this summer was to breed rodents(hamsters, rats, mice and gerbils). Most breeders give the animals this medicine typed stuff to cure them of anything like that before they sale them to pet stores. Hamsters get sick but they don't have anything major.
Reply:Diseases that pass from animals to people are called 'zoonoses'. I googled 'zoonoses + hamsters' and got this;


http://www.google.co.uk/search?hl=en%26amp;q=z...


I don't think you have much to worry about. Hamsters have been sold as childrens pets for generations, they don't carry many diseases and its rare for them to pass them to people.


Just take the usual precaustions, wash your hands after handling the animal or cleaning its cage.
Reply:No, its only wild mice that carry diseases anyway, not domesticated ones.
Reply:no but school s do
Reply:Hamster can carry some zoonotic diseases (illnesses that can be transmitted to other species, including humans). The one in recent headlines was the hamster that was infected with LCMV (Lymphocytic Choriomengingitis Virus) or Viral Meningitis. A woman had gotten a hamster infected with LCMV and died from an unrelated circumstance. Her organs were donated and four people received organ transplants. It seems the woman that died had caught LCMV from the hamster. In the average person, LCMV just gives the symptoms of a common cold. For a person with a weakened immune system, the virus is dangerous. Three of the people that received transplants died due to LCMV. The doctors were able to figure out what was happening and were able to save the fourth transplant patient. Health officials were able to trace it back to the hamster and then trace it back to the distributor. All the animals in the facility had to be destroyed. You may remember this a year and a half ago when most, if any, of the Petsmarts had small animals at all for several weeks.





There are a few other zoonotic dieases such as ringworm, Tyzzer's disease, salmonella, etc.. It is called Salmonellosis when a hamster is infected with salmonella. There are two types of salmonella that can infect hamsters. Once infected, the hamster will shed the virus in its feces. Hamsters can get over a salmonella infection on their own though once they recover, they are likely to become carriers of the disease and shed it in it's bedding for the rest of its life.





It is very important that you are always aware of hygiene when handling the hamster and cleaning its cage.





-Janice


http://www.holmdenhillhaven.com
Reply:no
Reply:well they are rodents so i would say yes


Small circular molecules of "extra" DNA in bacteria are called?

a. plasmids.





b. desmids.





c. pili.





d. F particles.





e. transferins

Small circular molecules of "extra" DNA in bacteria are called?
a. plasmids.
Reply:a - this is where the gene for insulin is added when producing genetically modified bacteria to make human insulin (for diabetics)
Reply:Plasmids - circular pieces of extra-chromosomal DNA
Reply:The answer is:


a) Plasmids.





Some more information about Plasmid :





A plasmid is a DNA molecule separate from the chromosomal DNA and capable of autonomous replication. It is typically circular and double-stranded. It usually occurs in bacteria, sometimes in eukaryotic organisms. Size of plasmids varies from 1 to over 400 kilobase pairs (kbp). There may be one copy, for large plasmids, to hundreds of copies of the same plasmid in a single cell, or even thousands of copies, for certain artificial plasmids selected for high copy number . Plasmids can be part of the mobilome, since they are often associated with conjugation, a mechanism of horizontal gene transfer.





The term plasmid was first introduced by the American molecular biologist Joshua Lederberg in 1952.


I need to know the name of bacteria or worm that comes from eating contaminated pork and affects the brain?

No one seems to have gotten this one right, so it seems I have to.





The name of the pork parasite that affects the brain is NOT trichinella but Taenia solium. It encysts in the brain and so causes a disease called neurocycticercosis.





Neurocysticercosis is treatable, and has nothing to do with mad cow or salmonella or trichinella.





More information at





http://www.emedicine.com/ped/topic1573.h...

I need to know the name of bacteria or worm that comes from eating contaminated pork and affects the brain?
trichina worm
Reply:botulism...the bacteria is clostridium botulinum
Reply:Taenia worm, salmonella poisoning
Reply:um... could it be the mad cow disease?
Reply:trichinella is the worm. its larvae can live in pork muscle (what we eat as meat) and cause an infection called trichinosis if you eat raw pork. Now the good news. Because of improved meat inspection standards in the USA, most pork products can be cooked to medium or medium well and still have virtually no chance of passing along this disease. Proper meat handling and storage is very important though,
Reply:Trichinella spiralis..a parasite ( worm)just what others said it can be found in pork muscles ( but not in canned goods). In canned foods, a bacteria Clostridium botulinum is the culprit.
Reply:Food safety is basically temperature and a time . Four hundred degrees for ten minutes will kill all bacteria known to man and make all food safe to eat. Larva from parasites are cooked. The temperature can be as low as boiling or two hundred and twelve degrees for thirty minutes to kill bacteria and yeast in all food. If the food has gone bad first the flavor may not be good enough to eat. It is best to wash off the salmonella slime then cook the chicken. Chemical poisons like herbicides may still be a poison. Heavy metals will still be there.
Reply:no need to worry, I think our nation has just about arrived at the no brainer era.


Don't antibacterial products kill the "good" bacteria making it difficult to fight off germs?

yes

Don't antibacterial products kill the "good" bacteria making it difficult to fight off germs?
no antibacterial soap is a good as regular soap


theres really no big differnce

camellia

What are some foods made from bacteria fermentation?

Could u also tell me the website too. thnx in advance =)

What are some foods made from bacteria fermentation?
1-Yogurt


2-Kefir


3-Sauerkraut


4-Pickled Ginger


5-Wine/Beer


6-Sourdough Bread


7-Olives


8-Lysine


9-Dill Pickles


9-Soy Sauce
Reply:Wine
Reply:i no abt curd


not sure abt bread


try yahoo search option
Reply:blue cheese
Reply:Yogurt, modern buttermilk, pickles, sourdough bread are made from lactobacillus. Cheeses contains various bacteria. There are tofu, soy bean and fish thats are made with bacteria.


Alcohol, bread and soy sauce are made from fungus, not bacteria
Reply:In other words, you want google. Most of this is common knowledge -- I honestly wouldn't need to look any of this up.
Reply:Cheese, and yogurt to name two.
Reply:1-Cheese


2-Kefir


3-Sauerkraut


4-Yogurt


5-Wine/Beer


6-Sourdough Bread


7-Olives


8-Lysine


9-Dill Pickles


9-Soy Sauce


10-Alcohol


If my toilet seat has less bacteria on it than my chopping board..?

Should I straddle the lavvy and dice my carrots at the same time?

If my toilet seat has less bacteria on it than my chopping board..?
Best be careful what you dice, you could do irrepairable damage :-S
Reply:That would be interesting....!
Reply:yeah its much cleaner that way?????!!!!
Reply:Yes and it will save time also
Reply:u r so funny! lol
Reply:That actually made me laugh which is hard to do. keep em comin! Comic relief is just around the corner and I would have tipped you for that!
Reply:definitely
Reply:sure ,but it's a sh#tter
Reply:y not might be better for ya
Reply:yes because its cleaner and saves time, well done for thinking of that!!!
Reply:Yes, I too have considered this predicament (every time I see the ad!) it is one of lifes most complex issues. Maybe they should get Paxman to lead a discussion on it. The public need to know the truth!
Reply:Clean your chopping board then.





Also remember there are good bacteria and bad bacteria - take your pick of where you will find each type.
Reply:Just include the chopping board with the washing up. And wash it before you chop, as well.
Reply:Don't be daft. All this means is that more bacteria are spread from food preparation than by going to the loo.The point of this remark is that you should be more concerned with the cleanliness of your kitchen equipment than of the toilet. Most people would do it the other way round.
Reply:Please be careful with the knife
Reply:Same as licking round the lavvy is more hygienic then biting your fingernails.
Reply:I totally agree, what a load of codswallop to try and sell anti bacterial cleaners which then increase allergies in kids!!





I think I will take your cue %26amp; prepare my coming dinner party from the comfort of my Lavvy!!!!!!!!! ( and yes I also use the word lavvy!)
Reply:multitasking rules!
Reply:Yes, but remember that there is even more bacteria under your fingernails, so wash them properly before you start.
Reply:..and you'll have somewhere handy to put your carrots before chopping them.
Reply:Lmao at the image that came to mind after reading your question!


At least you could cut out the middle man I suppose! But I would be very careful what you dice.... ouch
Reply:If you did that you might dice your onions too by mistake!


If i used your bog, the seat would probably melt !!!
Reply:Just make sure you don`t chop the wrong veg !
Reply:go for it just was hands before and after !
Reply:Some would say yes!! Although I don't like the idea of chopping my carrots on the toilet!! haha





Just make sure you clean your chopping board throughly or avoid wooden ones as they sometimes absorb the germs over time through the cuts and cracks.





I own a glass chopping board - just got to avoid using a mallet though! haha
Reply:just get rid of the chopping board and buy a new one
Reply:Ha ha ha!! No, don't do that please. Nor should you use the chopping board as a lavvy.
Reply:Why does everyone obsess with the cleanliness of toilet seats? Its not as if its touching you internally and the germs will transfer, they'll just go on your buttocks. Maybe i would be better posting this as a question?


I think that your idea sounds a good solution to the chopping board problem though.


What is the name of the bacteria in a termites stomach?

they are not in fact bacteria, but protists called Trichonympha.





these protozoa as well as other microbes help break down the lage amounts of cellulose eaten by your average termite

What is the name of the bacteria in a termites stomach?
http://www.microscopy-uk.org.uk/mag/wims... contains an excellent explanation, including identifying the protists which are the catalyst for the digestive process.
Reply:Termite gut symbionts:


spirotrichonympha bispira.
Reply:Bacterium - I'm not sure, but a Protozoan named Trichonympha lives in the gut of termites.... (It helps break down the cellulose constantly taken in by termites)
Reply:cheeky-stomach-livinghttp://www.nps.gov/archive/flfo/online_m...
Reply:There are a wide variety of different symbiotic organisms found in the guts of different termites.





Those that assist the termite in breaking down cellulose from plant material are generally flagellate protozoans, bacteria and yeast.





Each species of termite (and possibly even geographic populations) have different gut flora, and there is no single species of bacteria, yeast or flagellate that performs the endosymbiotic function for all termites.


If my toilet seat has less bacteria on it than my chopping board..?

Should I straddle the lavvy and dice my carrots at the same time?

If my toilet seat has less bacteria on it than my chopping board..?
Best be careful what you dice, you could do irrepairable damage :-S
Reply:That would be interesting....!
Reply:yeah its much cleaner that way?????!!!!
Reply:Yes and it will save time also
Reply:u r so funny! lol
Reply:That actually made me laugh which is hard to do. keep em comin! Comic relief is just around the corner and I would have tipped you for that!
Reply:definitely
Reply:sure ,but it's a sh#tter
Reply:y not might be better for ya
Reply:yes because its cleaner and saves time, well done for thinking of that!!!
Reply:Yes, I too have considered this predicament (every time I see the ad!) it is one of lifes most complex issues. Maybe they should get Paxman to lead a discussion on it. The public need to know the truth!
Reply:Clean your chopping board then.





Also remember there are good bacteria and bad bacteria - take your pick of where you will find each type.
Reply:Just include the chopping board with the washing up. And wash it before you chop, as well.
Reply:Don't be daft. All this means is that more bacteria are spread from food preparation than by going to the loo.The point of this remark is that you should be more concerned with the cleanliness of your kitchen equipment than of the toilet. Most people would do it the other way round.
Reply:Please be careful with the knife
Reply:Same as licking round the lavvy is more hygienic then biting your fingernails.
Reply:I totally agree, what a load of codswallop to try and sell anti bacterial cleaners which then increase allergies in kids!!





I think I will take your cue %26amp; prepare my coming dinner party from the comfort of my Lavvy!!!!!!!!! ( and yes I also use the word lavvy!)
Reply:multitasking rules!
Reply:Yes, but remember that there is even more bacteria under your fingernails, so wash them properly before you start.
Reply:..and you'll have somewhere handy to put your carrots before chopping them.
Reply:Lmao at the image that came to mind after reading your question!


At least you could cut out the middle man I suppose! But I would be very careful what you dice.... ouch
Reply:If you did that you might dice your onions too by mistake!


If i used your bog, the seat would probably melt !!!
Reply:Just make sure you don`t chop the wrong veg !
Reply:go for it just was hands before and after !
Reply:Some would say yes!! Although I don't like the idea of chopping my carrots on the toilet!! haha





Just make sure you clean your chopping board throughly or avoid wooden ones as they sometimes absorb the germs over time through the cuts and cracks.





I own a glass chopping board - just got to avoid using a mallet though! haha
Reply:just get rid of the chopping board and buy a new one
Reply:Ha ha ha!! No, don't do that please. Nor should you use the chopping board as a lavvy.
Reply:Why does everyone obsess with the cleanliness of toilet seats? Its not as if its touching you internally and the germs will transfer, they'll just go on your buttocks. Maybe i would be better posting this as a question?


I think that your idea sounds a good solution to the chopping board problem though.

lady slipper

What is the name of the bacteria in a termites stomach?

they are not in fact bacteria, but protists called Trichonympha.





these protozoa as well as other microbes help break down the lage amounts of cellulose eaten by your average termite

What is the name of the bacteria in a termites stomach?
http://www.microscopy-uk.org.uk/mag/wims... contains an excellent explanation, including identifying the protists which are the catalyst for the digestive process.
Reply:Termite gut symbionts:


spirotrichonympha bispira.
Reply:Bacterium - I'm not sure, but a Protozoan named Trichonympha lives in the gut of termites.... (It helps break down the cellulose constantly taken in by termites)
Reply:cheeky-stomach-livinghttp://www.nps.gov/archive/flfo/online_m...
Reply:There are a wide variety of different symbiotic organisms found in the guts of different termites.





Those that assist the termite in breaking down cellulose from plant material are generally flagellate protozoans, bacteria and yeast.





Each species of termite (and possibly even geographic populations) have different gut flora, and there is no single species of bacteria, yeast or flagellate that performs the endosymbiotic function for all termites.


Can you take or kill the bacteria from yogurt?

Kind of defeats the purpose...

Can you take or kill the bacteria from yogurt?
I believe the bactieria is already dead in anything that doesn't say "contains live cultures." I agree though, kinda defeats the purpose...
Reply:no you can't.
Reply:There is no bacteria in yoghurt unless it went bad. Yoghurt does contain live and active cultures, which is what produces the yoghurt. Kind of like the yeast in beer and breads are not bacteria.





If you are scare of them, just drink milk pureed with fruits - not the same health benefits, but we win some and we lose some.
Reply:Maybe if you microwaved it, but that would mess up the yogurt. And despite what the lady above me says, yes, it does contain bacteria, but usually dead unless it says otherwise. Those are bacterial "cultures" in the yogurt.





Still, why would you want to? That's the main health benefit of yogurt.





Edit: Now that I see why you want to know.... I still don't think it would work. Microwave is the only idea I have and that would probably mess up the yogurt. Maybe your science teacher would have an idea.
Reply:contains the bacterium acidophulus. which is what grows and eventually makes it sourer.


can kill it by heating it up.


the presence or lack of bacteria will not affect the growth of mold, which is different.
Reply:I think you are getting confused between bacteria, mold, enzymes, etc.


I am prostatitis.I have Streptococcus bacteria in my semen culture.Am I bacterial prostatits or non-bacterial?

Some doctors said bacterial,other non-bacterial

I am prostatitis.I have Streptococcus bacteria in my semen culture.Am I bacterial prostatits or non-bacterial?
Streptococcus is bacteria





You have bacterial prostatitis


Where do Streptococcus equi ssp equi bacteria live and thrive?

Primary reservoir of streptococci group A is in the oropharynx, skin, perineal area. group B is in adult genitals.

Where do Streptococcus equi ssp equi bacteria live and thrive?
horses (equine)





http://www.zoologix.com/horse/Datasheets...

dendrobium

How do cell walls deffer among bacteria, fungi, and plant?

Plant cell walls are made of cellulose, hemicellulose and pectin. Algae cell walls also contain glycoproteins, and other substances.


Fungal cell walls are made of chitin.


Bacterial cell walls are made of peptidoglycan (aka "murein").





Interestingly, archaebacteria have cell walls made of many different substances - but not peptidoglycan. They can be pseudopeptidoglycan, sulphated polysaccharides, glycoproteins, or proteins.


List the ingredients used to culture bacteria.?

There many different types of cultures and many many many types of bacteria. Depends on what are you looking for.





A microbiological culture, or microbial culture, is a method of growing a microbial organism to determine what it is, its abundance in the sample being tested, or both. It is one of the primary diagnostic methods of microbiology. A tool is often used to determine the cause of infectious disease by letting the agent multiply (reproduce) in predetermined media in laboratory.





The most common method of microbiological culture uses Petri dishes with a layer of agar-based growth medium in them to grow bacterial cultures. This is generally done inside of an incubator. Another method is liquid culture, where the bacteria are grown suspended in a liquid nutrient medium. Bottles of liquid culture are often placed in shakers in order to introduce oxygen to the liquid and maintaining the uniformity of the culture.





The term culture can also, though infrequently and informally, be used as a synonym for tissue culture, which involves the growth of cells or tissues explanted from a multi-cellular organism.

List the ingredients used to culture bacteria.?
A major constituent of the cell walls of certain red algae, especially members of the families Gelidiaceae and Gracilariaceae. Extracted for its gelling properties, it is one of three algal polysaccharides of major economic importance, the others being alginate and carrageenan. Agar is composed of two similar fractions, agarose and agaropectin, in which the basic unit is galactose, linked alternately α-1,3-(D-galactose) and β-1,4-(α-L-galactose).





Agar is prepared by boiling the algae in water, after which the filtered solution is cooled, purified, and dried. It is an amorphous, translucent material that is packaged in granules, flakes, bricks, or sheets. One of its chief uses is as a gelling agent in media for culturing microorganisms. It is also used in making confections, as an emulsifier in cosmetics and food products, as a sizing agent, as an inert carrier of drugs in medicine, and as a laxative.
Reply:Shortly to say (because there are so many various receipes for nutrient media), you need





- a source of carbon


- a source of nitrogen


- any growth factors if needed (amino acids, purines, pyrimidines, vitamins...)


- selective factors to eliminate competing organisms if needed (dyes, antibiotics...)





You also have to supply





- water


- beneficial pH


- oxygen (aerobes) / no oxygen (anaerobes)


- light (photosynthetic organisms) / no light (others)


- beneficial temperature





Please refer some comprehensive mb handbooks or cataloques by nutrient medium suppliers.
Reply:hm, everyone made it sound so complicated, i was just going to say agar (a nutrient medium!)


What are certain conditions that stop bacteria from growing out of control?

it's totally depends on the type of the bacteria!


1)sterilization:fungi is so sentitive to this condition.but if the bacteria is the kind that produce endospore then this method is not useful


2)pasteurization:kills all the bacterias except the one couses Query


3)filterization:useful for bacterias except Mycoplasma


4)Rays like UV X and Gamma


5)chemical Gases which are mutagenes like rays


6)chemical liquids

What are certain conditions that stop bacteria from growing out of control?
Killing it.
Reply:You could nuke the area and sterilize it.
Reply:under the condtion that you treat it with proper meds
Reply:A sanitary environment.
Reply:Heat the area to about 10000 Celsius. There, that should do the trick.
Reply:cooler,not hot or warm temps. Dryness, not moist. Light, not darkness. Keeping something clean, etc.
Reply:Both extreme heat and extreme cold.
Reply:temperature. keeping something cold enough(i.e. the fridge) keeps bacteria growth down. also, extreme heat does the same thing.(i.e. cooking meats above a certain temp actually kills the bacteria).
Reply:the carrying capacity of the system that they are in. nutrients, antibiotics, the actual organism, the temperature, pH, competition, osmotic gradients, the concentration of the organism and the list continues!
Reply:Taking Medications suggested by your doctors.





Also you can desinfected it by using lotions, creams, and some kind of water usable.
Reply:bleach.


What is the name of the bacteria that causes acne?

Propionibacterium acne.


I think that was the answer you wanted. However, when you actually send a q-tip full of pimple ooze to the lab, it grows far more that just propionii. There would be normal skin flora, which means bacteria that grows on your skin NORMALLY

What is the name of the bacteria that causes acne?
staph aureus.
Reply:"Oil secretions are said to build up beneath the blocked pore, providing a perfect environment for the skin bacteria Propionibacterium acnes and the lipophilic (oil/lipid-loving) yeast Malassezia[citation needed] to multiply uncontrollably."

broadleaf

How can we get energy from bacteria ; electrical energy?

try cultivating bacterium that produce flammable gas use gas to fuel boiler generator?

How can we get energy from bacteria ; electrical energy?
...watch the matrix...


How long does it take for bacteria to grow on agar?

I mean enough to report on, not the full amount it will be. Please give me the min. amont of days. Thanks a bunch

How long does it take for bacteria to grow on agar?
It depends on the type of bacteria, but in general you should see some growth within 2 days. Be careful about reporting on your growth before 2 days because you may not see much growth and it would be hard to make any conclusions.





If you're in a hurry to report on your data and you have control over where your plates are, then you can speed up growth by placing them in a warm dark place, with high humidity.


Explain the role of nitrogen fixing bacteria to providing soil fertility?

i am pretty sure that it converts atmospheric nitrogen N2 to NH3 (fixed nitrogen) that the plants use for growth


I'll hav e a look in my text books and get back to you





ok; so i checked it out and what i stated above is just part of the nitric acid cycle. this part is N2 Fixation and there are 3 types, aerobic (by organisms such as Cyanobacterium) anaerobic (eg Clostridium), there is also symbiotic ( eg Rhizobium)


There are also other processes in the Nitrogen Cycle such as Nitricifation (NH4+ to NO3-) and Dentrification (NO3- to N2)


you should do a little research I cannot explain it all here it is a very involed concept.


but primarily Nitrogen is an energy source for plants and bacteria make this available to plants by converting it to forms that can be used by the plant eg N2 to NH3

Explain the role of nitrogen fixing bacteria to providing soil fertility?
you just did.


Is strep throat caused by a bacteria or a virus?

Strep is a bacteria, and is treatable by antibiotics. If you suspect you have strep, you need to see your doctor and have a step test done. While the doctor is waiting for the results, he will prescribe antibiotics. Make sure you take the entire course of antibiotics, because if the bacteria is not completely gone, you can find yourself very sick. Step can cause many other problems, like rheumatic fever.

Is strep throat caused by a bacteria or a virus?
bacteria
Reply:Bacteria. Group A streptococcus, to be specific.
Reply:bacteria

night jasmine

What are the "survival strategies of bacteria in natural environment"?

pls include link @ websites...tq

What are the "survival strategies of bacteria in natural environment"?
Mutation.


Spores formation.





http://en.wikipedia.org/wiki/Bacteria
Reply:different bacteria have different strategies. some cld even be opportunistics such as survivng the extreme conditon in nature (pH, temperature, salinity and etc.)








p/s : Googles could do some trick.


What is meant by "green sulphur bacteria" ?

Its a bacteria that uses sulfide instead of CO2, kind of odd actually. They photosynthesize like plants, but instead of creating O2, they produce sulfur as waste.

What is meant by "green sulphur bacteria" ?
The green sulfur bacteria (Chlorobiaceae) are a family of phototrophic bacteria. No other bacterial families are known to be closely related to them, and they are accordingly placed in their own phylum (Chlorobi). The phylum is most closely related to Bacteroidetes.
Reply:Green sulfur bacteria are a distinct group of anaerobic bacteria that utilize H2S (hydrogen sulfide) as an electron donor and produce Sulfate.





They can be fully autotrophic or photoheterotrophic, meaning they take up some fixed carbon in the light.





they are similar to purple sulfur bacteria except that the sulfur they produce resides outside the cell.
Reply:The green sulfur bacteria (Chlorobiaceae) are a family of phototrophic bacteria. No other bacterial families are known to be closely related to them, and they are accordingly placed in their own phylum (Chlorobi). The phylum is most closely related to Bacteroidetes.





Green sulfur bacteria are generally nonmotile (one species has a flagellum), and come in spheres, rods, and spirals. Their environment must be anaerobic (oxygen-free), and they need light to grow. They engage in photosynthesis, using bacteriochlorophylls c, d, and e in vesicles called chlorosomes attached to the membrane. They use sulfide ions as electron donor, and in the process the sulfide gets oxidized, producing globules of elemental sulfur outside the cell, which may then be further oxidized. (By contrast, the photosynthesis in plants uses water as electron donor and produces oxygen.)





A species of green sulfur bacteria has been found living near a black smoker off the coast of Mexico at a depth of 2,500 meters beneath the surface of the Pacific Ocean. At this depth, the bacteria, designated GSB1, lives off the dim glow of the thermal vent since no sunlight can penetrate to that depth.


What is the pH tolerance of bacteria compared to Yeasts?

Bacteria usually hang in between 5 and 8, but there are of course extreme exceptions. Yeast exhibit considerable tolerance for extremes of Ph.


What is the main difference between bacteria and most other living cells?

Bacteria as mentioned are prokaryotic, so have no nucleus and their DNA is contained in a mass of tangled strands. Many bacteria also contain plasmids, rings of parasitic DNA that are manipulated for many genetic engineering applications. Bacterial cells are generally much smaller than plant or animal cells and do not contain compartmentalized organelles(i.e mitochondria, endoplasmic reticulum, chloroplasts) as mentioned above. This makes them capable of reproducing and metabolizing much more quickly than larger cells.

What is the main difference between bacteria and most other living cells?
Well.....bacteria are prokaryotic (or single-celled) organisms, where as most other living cells are eukaryotic (or many-celled) organisms. I'm pretty sure that's the main difference.





Ok, so I was wrong about eukaryotes being only many-celled organisms. But your question WAS what is the difference between bacteria and most other living cells and that difference is that bacteria are prokaryotic. So BLAH!
Reply:No no no. There are many single celled eukariotic organisms. Yeast are one example. Prokariotes do not have organells, nuclii, or chromosomes whereas eukariotic cells do.

frangipni

One good use or consequence of bacteria?

I need this 4 my biology class, so i need this answer ASAP

One good use or consequence of bacteria?
One good use for bacteria is that it can supply your body with vitamin K. Your gut is colonized by bacteria that as a by-product of their metabolism produce vitamin K. Without this colonization you could have bleeding problems.
Reply:Cheese. Here's a government link:


http://www.newton.dep.anl.gov/askasci/bi...


What are the five genera of bacteria that form endospore?

Bacillus, Clostridium, Desulfotomaculum, Sporolactobacillus and Sporosarcina.


Good luck with whatever microbiology project this comes from!!

What are the five genera of bacteria that form endospore?
Five genera of spore forming bacteria:


1. Bacillus


2. Clostridium


3. Desulfotomaculans


4. Sporolactobacillus


5. Sporpsarcina





or from wikipedia:


Endospore-forming bacteria


Examples of endospore-forming bacteria include the genera:





1. Bacillus


2. Clostridium


3. Desulfotomaculum


4. Sporolactobacillus


5. Sporosarcina


6. Thermoactinomyces


How does antibiotic resistance arise in bacteria? Or pesticide-resistance in insects too?

It's basically evolution. Let's say that you spray 1,000 bugs, for instance. 998 of them die, but for some strange reason, a quirk in their DNA, two of them survive. Those two are slightly resistant to the pesticides. They manage to reproduce, and a slightly higher percentage, let's say, 10 in 1,000, inherit the resistant genes. If you spray again, only these 10 will survive and reproduce. As the generations continue, more and more bugs with resistance appear. The more there are, the more selective it becomes, until only the most resistant bugs can reproduce and pass on their superior genes. After several generations, a pesticide-resistant group of insects has developed. The same process happens with bacteria.

How does antibiotic resistance arise in bacteria? Or pesticide-resistance in insects too?
The resistance arises the same way in both bacteria and insects. Whenever an antibiotic or pesticide is applied, it initially kills 99.9% of the organism. That seems like in would do the job, right?





But, it is the .1% that manage to survive that repopulate the environment with more resistant survivors. After a while the entire population is resistant and new antibiotics or pesticides have to be found.





It's an ongoing battle.
Reply:Selection. Natural selection if you'd like to call it that.


Neither artificial nor natural selection are perfect terms, but the mechanism is the same.
Reply:It's evolution , baby! These organisms are not identical clones of each other. Processes of mutation have created some variety in their genetic makeup, and sexual reproduction can add more variety yet.





When you spray (or dose) them, they naturally aren't all equally affected. Some will succumb very quickly, some will succumb slowly, and there may be some that won't even die from it. When these survivors breed, the next generation has a proportionally higher frequency of the genes that provided the mechanism for resistance, because the resistant ones of the first gen had a better chance of surviving to reproduce than the vulnerable ones.





When sprayed a second time, a greater fraction of the new generation would be expected to survive. And so on with further sprayings, until that chemical is no longer effective against them.
Reply:think of it on a larger scale. some people get the flu and some don't. something in their genetic code makes their immune system more affective against the flu virus.





same with bacteria. some bacteria is killed off by antibiotics, but not all. the ones that survive have something in their genetic code that makes it harder to kill. so we use a stronger antibiotic and most will be killed but not all.





basically we weed out the week bacteria, and the stronger leftovers come back with a vengeance, then we kill off most of those and the stronger left overs come back even stronger.





hope this helps!


Can someone explain how to subculture bacteria from colonies on agar plate?

You need to pick (in a sterile manner) some of the bacteria spread from the first agar plate and streak them onto a new agar plate, taking care of turning the plate of a few degrees every so often. To be sure that you are in limiting conditions, which will allow you to end up with individual colonies, you could streak the bacteria a second and third time on sequential plates - therefore performing a "serial dilution" of your bacterial titer. So at least in one of them you will have few enough bacteria to grow sparse and make individual colonies.


You may find a diagram on how it is done here:


http://loudoun.nv.cc.va.us/vetonline/vet...

Can someone explain how to subculture bacteria from colonies on agar plate?
Use a sterile loop to get a sample from the bacteria and streak this sample on to another sterile petri plate.

bleeding heart